Title | High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | B Polyak, I Fishbein, M Chorny, I Alferiev, D Williams, B Yellen, G Friedman, and RJ Levy |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 105 |
Issue | 2 |
Start Page | 698 |
Pagination | 698 - 703 |
Date Published | 01/2008 |
Abstract | A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls. |
DOI | 10.1073/pnas.0708338105 |
Short Title | Proceedings of the National Academy of Sciences of the United States of America |